Preyalence of Cexiella burnetii Infection in Dairy Cattle with Repreductiye Disorders

,s.BsTRAcT, The prevalence of CtJ.aietla hurnetii infection in 207 cattle with repreductive disorders was studied by using an indireet immunofiuorescence <IF) tcst, nested polymerase chain reaction {PCR) and isolatien, IF antibodies te phase I and phase II antigens ef C, httrnetii were found in l22 (58.9%) and I25 <60.4%) of the sefa. respeclivcly, and PCR-positives svere found in 8 (3.9%) of thc sera and in 51 {24.6%} of the milk samples. In additiofi, C. hurnetii was lsolated from Si <24.69b) of the rni[k sampLes by inoculating IaboratoTy mice. The resv]ls indicate that the [F test plus PCR are usef"I in thc diagnosis of bovine eexiellosis. It is difflcult to cleny that dairy cattle with Teproductive disorders weuld be one of the irnportant rcscrveirs of C. httrnetii responsibte for infection in both animal and huTnan